The Austrian and Croatian capitals were linked by a daily direct train, but this train now commences its journey in Graz, so a connection is now required there when travelling from Wien/Vienna
Alternative slower routings are available with connections in Villach, but the morning departure from Wien/Vienna gives an opportunity to enjoy more than 8 hours of stunning scenery year round.
So if time is on your side, it is highly recommended!
Despite the need to now make connections by day, travelling overnight isn't feasible on this route.
When travelling between most cities by train there is only one logical option, though on other journeys there is a choice between different trains or alternative routes. If different options are available you can use the info to decide which is best for you.
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This is one of more than 100 train travel guides available on ShowMeTheJourney, which will make it easier to take the train journeys you want or need to make. As always, all images were captured on trips taken by ShowMeTheJourney.