Making long journey by day from Amsterdam to Prague / Praha by train is easier than you may imagine.
Only one connection is required in the magnificent main station in Berlin and the time available between trains should ensure a stress-free transfer.
Making the journey overnight isn't feasible, there are now no night trains between Amsterdam and Berlin or between Berlin and Prague.
When travelling between most cities by train there is only one logical option, though on other journeys there is a choice between different trains or alternative routes. If different options are available you can use the info to decide which is best for you.
Mon - Sat = 2 x connections per day
Sunday = 1 x connection per day
Change trains in: Berlin
Make this journey and you will have around 1hr 47mins to transfer between trains in Berlin, so making the connection into the train on to Prague/Prag/Praha is virtually guaranteed.
Berlin Hbf and Berlin Hbf (tief) are the same station, the 'tief' platforms are at a lower level.
If the train from Amsterdam arrives on time, an option is to take the U-Bahn to the area around the Brandenburg Gate and enjoy a brief glimpse of Berlin between trains.
Though Berlin Hbf is packed with travellers facilities, with numerous food and drink outlets in which to wait for the onward journey.
There is also a mall area which is handy for picking up travel essentials
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This is one of more than 100 train travel guides available on ShowMeTheJourney, which will make it easier to take the train journeys you want or need to make. As always, all images were captured on trips taken by ShowMeTheJourney.